Alpha-amylase (Yim et al., 2016)

Technical Data

Microorganism name	Corynebacterium glutamicum
Target proteins	Alpha-amylase (Yim et al., 2016)
Wild-type or GMO	GMO (Yim et al., 2016)
Production mode (intracellular/extracellular)	Extracellular (Yim et al., 2016)
Protein yield (g/L or g/g?)	0.783 g/L (Yim et al., 2016)
Temperature used in study	30°C (Yim et al., 2016)
pH used in study	pH 7.0 (Yim et al., 2016)
C & N source	Glucose, yeast extract, urea (Yim et al., 2016)
Regulatory status in Europe	Not allowed
Regulatory status in other parts of the world	No FDA GRAS approval in US, not allowed in Canada
Companies	NA
Publications/references	Yim, S. S., Choi, J. W., Lee, R. J., Lee, Y. J., Lee, S. H., Kim, S. Y., & Jeong, K. J. (2016). Development of a new platform for secretory production of recombinant proteins in Corynebacterium glutamicum. Biotechnology and Bioengineering, 113(1), 163–172. https://doi.org/10.1002/bit.25692 Haas, T., Graf, M., Nieß, A., Busche, T., Kalinowski, J., Blombach, B., & Takors, R. (2019). Identifying the Growth Modulon of Corynebacterium glutamicum. Frontiers in Microbiology, 10. https://doi.org/10.3389/fmicb.2019.00974 Lee, M. J., & Kim, P. (2018). Recombinant Protein Expression System in Corynebacterium glutamicum and Its Application. Frontiers in Microbiology, 9. https://doi.org/10.3389/fmicb.2018.02523 Lee, S. M., & Jeong, K. J. (2022). Advances in Synthetic Biology Tools and Engineering of Corynebacterium glutamicum as a Platform Host for Recombinant Protein Production. Biotechnology and Bioprocess Engineering, 28(6), 962–976. https://doi.org/10.1007/s12257-022-0219-1 Zhang, B., Jiang, Y., Li, Z., Wang, F., & Wu, X. (2020). Recent Progress on Chemical Production From Non-food Renewable Feedstocks Using Corynebacterium glutamicum. Frontiers in Bioengineering and Biotechnology, 8. https://doi.org/10.3389/fbioe.2020.606047 Bello, P. R., Anzola, A. G., Becerril, A. O., & Gutiérrez, D. F. (2025). Evaluation of the Potential of Corynebacterium glutamicum ATCC 21492 for L-Lysine Production Using Glucose Derived from Textile Waste. Fermentation, 11(6), 355. https://doi.org/10.3390/fermentation11060355 Liu, X., Zhang, W., Zhao, Z., Dai, X., Yang, Y., & Bai, Z. (2016). Protein secretion inCorynebacterium glutamicum. Critical Reviews in Biotechnology, 37(4), 541–551. https://doi.org/10.1080/07388551.2016.1206059 Eastham, J. L., & Leman, A. R. (2024). Precision fermentation for food proteins: ingredient innovations, bioprocess considerations, and outlook — a mini-review. Current Opinion in Food Science, 58, 101194. https://doi.org/10.1016/j.cofs.2024.101194 Follmann, M., Ochrombel, I., Krämer, R., Trötschel, C., Poetsch, A., Rückert, C., Hüser, A., Persicke, M., Seiferling, D., Kalinowski, J., & Marin, K. (2009). Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis. BMC Genomics, 10(1). https://doi.org/10.1186/1471-2164-10-621